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Objective:To analyze the preoperative and postoperative color Doppler ultrasonographic features of Abernethy malformation in children, and to investigate the value of ultrasound diagnosis of Abernethy malformation and postoperative complications.Methods:A retrospective analysis was performed on the clinical and ultrasound data of twelve cases of Abernethy malformation confirmed by surgical treatment in the General Surgery Department of the Children′s Hospital Affiliated to Capital Institute of Pediatrics from February 2017 to November 2021. A comparison was made between preoperative ultrasound and intraoperative portal vein angiography after shunt ligation to explore the accuracy of preoperative ultrasound in diagnosing Abernethy malformation; The common location of thrombosis after shunt ligation was summarized by comparing postoperative ultrasound with CT angiography.Results:Preoperative ultrasonography showed no main portal vein or cable shape in 9 cases, and they were diagnosed as probable Abernethy type Ⅰ; The main portal vein was narrow in 3 cases, and they were diagnosed as Abernethy type Ⅱ. The main portal veins of 11 case were developing and they were confirmed as Abernethy malformation type Ⅱ by portal vein angiography after blocking of portosystemic shunt; the main portal vein of 1 case was not developing which was confirmed as Abernethy type Ⅰ. The classification accuracy of preoperative ultrasound diagnosis of Abernethy malformation was 33.3%. Preoperative ultrasound diagnosis of shunt vessel location: the coarse inferior mesenteric veins of 7 cases flowed into the iliac vein, the coarse inferior mesenteric vein of 1 case flowed into the inferior vena cava, splenic vein and superior mesenteric vein converged and flowed into inferior vena cava in 2 cases, splenic vein and left renal vein communicated in 2 cases. The location of shunt vessels diagnosed by portal vein X-ray angiography was basically consistent with preoperative ultrasonography. At the same time, inferior mesenteric vein shunt combined with tortuous and dilated vein network on colorectal surface was observed. After ligation of shunt vessels, all of shunt vessels were occluded or thrombolized in varying degrees.Splenic vein retropancreatic segment of three cases occured secondary thrombosis, and one case of blocked portal vein occured secondary cavernous change. All the thrombi were confirmed by CT angiography.Conclusions:①The main portal vein of Abernethy malformation type Ⅱ is tenuous, and is easily misdiagnosed Abernethy malformation type Ⅰ by preoperative ultrasound examination; ②Preoperative ultrasound can determine the location of Abernethy malformed shunt vessels; ③The shunt between the inferior mesenteric vein-iliac vein/inferior vena cava should be emphatically explored in children with recurrent hematochezia; ④Postoperative ultrasound can detect portal vein thrombosis early and provide help for clinical anticoagulant therapy.
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Glioma-associated microglial cells, a key component of the tumor microenvironment, play an important role in glioma progression. In this study, the mouse glioma cell line GL261 and the mouse microglia cell line BV2 were chosen. First, circadian gene expression in glioma cells co-cultured with either M1 or M2 microglia was assessed and the exosomes of M2-polarized and unpolarized BV-2 microglia were extracted. Subsequently, we labeled the exosomes with PKH67 and treated GL261 cells with them to investigate the exosome distribution. GL261 cell phenotypes and related protein expression were used to explore the role of M2 microglial exosomes in gliomas. Then a specific miR-7239-3p inhibitor was added to verify miR-7239-3p functions. Finally, the mouse subcutaneous tumorigenic model was used to verify the tumorigenic effect of M2 microglial exosomes in vivo. Our results showed that in gliomas co-cultured with M2 microglia, the expression of the BMAL1 protein was decreased (P < 0.01), while the expression of the CLOCK protein was increased (P < 0.05); opposite results were obtained in gliomas co-cultured with M1 microglia. After treatment with M2 microglial exosomes, the apoptosis of GL261 cells decreased (P < 0.001), while the viability, proliferation, and migration of GL261 cells increased. Increased expression of N-cadherin and Vimentin, and decreased E-cadherin expression occurred upon treatment with M2 microglial exosomes. Addition of an miR-7239-3p inhibitor to M2 microglial exosomes reversed these results. In summary, we found that miR-7239-3p in the glioma microenvironment is recruited to glioma cells by exosomes and inhibits Bmal1 expression. M2 microglial exosomes promote the proliferation and migration of gliomas by regulating tumor-related protein expression and reducing apoptosis.
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Objective:To discuss the ultrasonographic features of ganglioneuroma(GN), ganglioneuroblastoma-intermixed(GNBi), ganglioneuroblastoma-nodular(GNBn), neuroblastoma(NB) in children and to improve the ultrasound diagnostic accuracy of four kinds of neuroblastic tumors.Methods:Two hundred and seventy-nine patients with neuroblastic tumors(23GN, 44GNBi, 86GNBn, 126NB) confirmed by surgery and pathological diagnosis in Children′s Hospital Affiliated to Capital Institute of Pediatrics from June 2014 to February 2019 were retrospectively analysed. The ultrasonographic data were summarized and compared with pathological results.Results:The median age were 6.9 years in GN group, 3.9 years in GNBi group, 3.0 years in GNBn group and 2.0 years in NB group. The median values of serum neuron-specific enolase(NSE) were 6.3 μg/L in GN group, 9.6 μg/L in GNBi group, 22.6 μg/L in GNBn group and 40.7 μg/L in NB group respectively. There was no distant metastasis of GN, while 9% GNBi, 26% GNBn and 36% NB had distant metastasis; GN and GNBi group had no invasion to adjacent tissues and organs, while 9% GNBn and 15% NB lesions had invasion to adjacent tissues and organs; 91% GN, 70% GNBi, 58% GNBn lesions had complete capsule, while 44% NB lesions had incomplete capsule and 28% NB had no capsule; 96%GN, 57%GNBi lesions were round or oval, while 57%GNBn, 60%NB lesions showed irregular shape; 96%GN showed homogeneous hypoechoic, 75% GNBi had "cloudy" inhomogeneous echoe, while hyperechoic nodules were seen in 59% GNBn and 75% NB lesions; 65%GN lesions contained discrete and punctate calcifications, while 27%GNBi, 29%GNBn, 25%NB lesions contained coarse calcifications; 100%GN, 91%GNBi, 91%GNBn lesions had little to moderate blood flow, while 33%NB lesions had abundance blood flow; 4%GN, 41%GNBi, 51%GNBn, 49%NB lesions surrounded blood vessels; 13%GN, 5%GNBi, 6%GNBn, 8%NB lesions had spinal canal invasion; GN had no lymph node metastasis, but 48%GNBi, 59%GNBn, 56%NB lesions had lymph node metastasis.Conclusions:The ultrasonographic characteristics of GN are largely different from GNBi, GNBn and NB; There are some differences in ultrasonographic features of GNBi, GNBn and NB, but some of them are difficult to identify.
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Objective:To assess the diagnostic value of ultrasound for pediatric pancreatitis in different ages via summarizing the ultrasonographic characterizations of pediatric acute pancreatitis (AP) and chronic pancreatitis (CP) patients in different ages. Methods:From January 2013 to June 2018, ultrasonographic images and related clinical data of 81 AP and CP children hospitalized in Children′s Hospital of Capital Institute of Pediatrics were retrospectively analyzed. Patients were divided into two age groups: 0-5 and 5-18 years (23 and 58 patients respectively). The ultrasonography of 23 little children(≤5 years old) and 58 big kids (>5 years old) was compared.Results:All 23 little children were diagnosed as AP, including recurrent AP. 41 of 58 big kids (71%) were diagnosed as AP, and 17 (29%) were as CP. There were no significant differences between AP little children and AP big kids on the incidence of abnormal findings in pancreatic ultrasound, enlargement of pancreas, increased echogenicity of pancreas, heterogeneous pancreas, dilation of pancreatic duct, ductal calculus, calcification, unsmooth capsule of pancreas, increased echogenicity of peripancreatic tissue and peripancreatic fluid collection, pseudocyst, common bile duct dilatation and portal vein system thrombosis. Diffuse enlargement of the pancreas was seen more frequently in little children than big kids (88% vs 52%), while localized pancreatic enlargement occurred more often in big kids than little children (48% vs 13%), and both the differences were statistically significant ( P<0.05). Ultrasonography indicated that 9 (39%) little children with common bile duct dilatations were eventually diagnosed as choledochal cysts by surgery, while 6 (15%) of the 8 big kids with common bile duct dilatations were confirmed to be choledochal cysts by surgery, and the difference on the incidence of choledochal cysts was statistically significant ( P<0.05). All the CP patients were big kids. 47%(8/17) of CP big kids had parenchymal atrophy, 100%(17/17) had heterogeneous pancreas, 82%(14/17) had pancreatic duct dilation, 29%(5/17) had pseudocyst, 18%(3/17) had pancreatic calcification and ductal calculus. Conclusions:In ultrasonography for little children, the presence of congenital anatomy abnormalities, especially choledochal cyst, which can cause pancreatitis potentially, should be paid special attention to. In ultrasonography for big kids, overall scanning of the pancreas is necessary and the lesions in pancreatic body and tail should be carefully checked. Additionally, CP should be not be neglected in big kids.
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Objective@#To assess the diagnostic value of ultrasound for pediatric pancreatitis in different ages via summarizing the ultrasonographic characterizations of pediatric acute pancreatitis (AP) and chronic pancreatitis (CP) patients in different ages.@*Methods@#From January 2013 to June 2018, ultrasonographic images and related clinical data of 81 AP and CP children hospitalized in Children′s Hospital of Capital Institute of Pediatrics were retrospectively analyzed. Patients were divided into two age groups: 0-5 and 5-18 years (23 and 58 patients respectively). The ultrasonography of 23 little children(≤5 years old) and 58 big kids (>5 years old) was compared.@*Results@#All 23 little children were diagnosed as AP, including recurrent AP. 41 of 58 big kids (71%) were diagnosed as AP, and 17 (29%) were as CP. There were no significant differences between AP little children and AP big kids on the incidence of abnormal findings in pancreatic ultrasound, enlargement of pancreas, increased echogenicity of pancreas, heterogeneous pancreas, dilation of pancreatic duct, ductal calculus, calcification, unsmooth capsule of pancreas, increased echogenicity of peripancreatic tissue and peripancreatic fluid collection, pseudocyst, common bile duct dilatation and portal vein system thrombosis. Diffuse enlargement of the pancreas was seen more frequently in little children than big kids (88% vs 52%), while localized pancreatic enlargement occurred more often in big kids than little children (48% vs 13%), and both the differences were statistically significant (P<0.05). Ultrasonography indicated that 9 (39%) little children with common bile duct dilatations were eventually diagnosed as choledochal cysts by surgery, while 6 (15%) of the 8 big kids with common bile duct dilatations were confirmed to be choledochal cysts by surgery, and the difference on the incidence of choledochal cysts was statistically significant (P<0.05). All the CP patients were big kids. 47%(8/17) of CP big kids had parenchymal atrophy, 100%(17/17) had heterogeneous pancreas, 82%(14/17) had pancreatic duct dilation, 29%(5/17) had pseudocyst, 18%(3/17) had pancreatic calcification and ductal calculus.@*Conclusions@#In ultrasonography for little children, the presence of congenital anatomy abnormalities, especially choledochal cyst, which can cause pancreatitis potentially, should be paid special attention to. In ultrasonography for big kids, overall scanning of the pancreas is necessary and the lesions in pancreatic body and tail should be carefully checked. Additionally, CP should be not be neglected in big kids.
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OBJECTIVE：To establish QAMS method for simultaneou s determination of 7 effective components in Yao medicine Yueli yaomi spray ，such as α-cyperone，α-pinene，β-pinene，limonene，β-elemene，caryophyllene oxide and ligustilide ， so as to provide method reference for the quality control of the preparation. METHODS ：GC method was adopted. The determination was performed on DB- 1701P capillary column ，using nitrogen as carrier gas. The temperature of the hydrogen flame ion detector was 240 ℃. The temperature was programmed ，the inlet temperature was 240 ℃，the injection volume was 1 μL and the split ratio was 20 ∶ 1. Using limonene as internal reference ，the relative correction factors of other 6 components were calculated，the contents of them were calculated with relative correction factors ，and then compared with the results of internal standard method （using naphthalene as internal standard ）. RESULTS ：The mass concentration linear range of α-cyperone， α-pinene，β-pinene，limonene，β-elemene，caryophyllene oxide and ligustilide were 0.008 9-1.110 0，0.028 3-3.540 0，0.020 5- 2.560 0，0.023 0-2.880 0，0.016 3-2.035 0，0.013 1-1.640 0，0.008 3-1.040 0 mg/mL（all r＞0.999 0）；the limits of quantification were 0.005 6，0.013 1，0.011 4，0.018 6，0.010 8，0.008 9，0.004 5 mg/mL；the detection limits were 0.001 9，0.004 1，0.003 7， 0.006 2，0.003 5，0.002 9，0.001 5 mg/mL；RSDs for precision ，stability（24 h），and repeatability tests were all less than 2％ （n＝5 or n＝6）； the average recoveries were 98.48％ ， 014） 101.37％，97.96％，99.80％，102.79％，97.77％，102.14％， and RSDs were all lower than 2％（n＝9），respectively. The average relative correction factors of α-cyperone，α-pinene， β-pinene，β-elemene，caryophyllene oxide and ligustilide were 1.045 8，0.621 0，0.488 5，0.382 9，0.708 9，0.956 9 respectively，and the RSDs were all lower than 2％（n＝6）. There wa s no statistical significance in contents of 7 components between QAMS method and internal standard method （P＞0.05）. CONCLUSIONS ：The established QAMS method is simple ， accurate，stable and reproducible ，and can be used for simultaneous determination for 7 components in Yueli yaomi spray.
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Objective The nursing staff's output and journals distribution of core scientific journals papers were analyzed, providing evaluation data and a decisive basis for scientific research management. Methods A retrospective analysis was performed to the publication of core journals papers of nursing staff in 2013-2015 based on the publication from different nursing positions , the ranking of published nursing papers from different departments and different journals by using analysis method of bibliometric. Results About 1550 nursing papers were published , of which the number of authors whose paper was published in core journals was 216 , the number of papers published in core journals was 378, and 38 authors published more than 3 papers (17.6%). The core journals papers published rate in different position was statistically significant (P<0.01). The papers published journals were concentrated in nursing class. Dominated the first 3 places in the list of core journals paper number of departments were department of nursing , cancer center and operation room, respectively. Conclusion In order to improve the nursing scientific research level of nursing staff, it is necessary to establish a theoretical system of scientific knowledge training , formulate a long-term effective mechanism in paper management , focus on scientific research talent introduction and training and mobilize the nursing research enthusiasm of nursing staff.
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Objective To explore the clinical curative effect of recombinant human endostatin injection combined with chemotherapy in the treatment of patients with advanced gastric cancer and malignant ascites,and its influence on the quality of life.Methods 62 patients with advanced gastric cancer from July 2012 to July 2015,were randomly divided into observation group (31 cases)and control group (31 cases).The control group was treated with FOLFOX6 chemotherapy,the observation group was given recombinant human endostatin injection on the basis of the treatment of the control group.The two groups were treated for 3 weeks.The curative effect,QOL score,Karnofsky score,the change of serum CEA and CA19 -9 levels and drug adverse reaction incidence before and after treatment were compared in the two groups.Results The RR of the observation group was higher than that of the control group (54.84 vs 29.03%,χ2 =4.239 3,P 0.05).Conclusion The clinical efficacy of recombinant human endostatin injec-tion combined with malignant ascites in the treatment of patients with advanced gastric carcinoma is significant,and can significantly improve the quality of life of patients,has the important research value.
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Objective To investigate the imaging manifestations of congenital intestinal malrotation. Methods The clinical data of 92 infants with congenital intestinal malrotation admitted from January 1993 to December 2012 were studied retrospectively. All the 92 cases enrolled in this study were diagnosed based on imaging examinations and confirmed surgically. The imaging features of four examinations including plain abdominal radiography, upper gastrointestinal radiography, lower gastrointestinal angiography and ultrasound examination were analyzed. Results The percentage of the infants who presented with the initial clinical symptoms of vomit, blood stool and abdominal dilatation was 87.0%(80/92), 3.3%(3/92) and 1.1%(1/92), respectively, and eight cases [8.7%(8/92)] received prenatal ultrasound, which showed intestinal malrotation. Clinical examination showed no abdominal abnormalities in 68 (73.9%) cases. Seventy-seven cases underwent plain abdominal radiography, revealing intestinal obstruction in 31 cases. Thirty-six cases underwent upper gastrointestinal radiography, which revealed malrotation in 14 cases, and duodenal complete/incomplete obstruction or stenosis in 19 cases. Twenty-two cases underwent lower gastrointestinal angiography, and all of them were diagnosed as malrotation. Seventy-nine cases were examined with abdominal ultrasonography, and 58 cases were diagnosed as malrotation. Conclusions Rational selection of imaging examinations can improve the preoperative diagnosis. When congenital intestinal malrotation is suspected, abdominal ultrasound should be done as the first optional examination. At the same time, plain abdominal radiography should be selected accordingly. When intestinal malrotation with or without midgut volvulus is suggested, surgery should be performed as early as possible. When the diagnosis is not clear, upper or lower gastrointestinal radiography should be done.
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Cardiovascular disease caused by atherosclerosis has been the world′s primary cause of death. Studies have shown that early intervention in preclinical stage of atherosclerosis among children can reverse the disease and ultimately reduce the morbidity of atherosclerosis related diseases after they grow up,which is crucial to reduce and eliminate the harm of atherosclerosis.Now,the risk factors and detection methods of children′s preclinical atherosclero-sis were reviewed.
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Objective To evaluate the value of ultrasound in the diagnosis of congenital intestinal malrotation and midgut vovulus.Methods The findings of ultrasound of 161 children with clinically suspected intestinal malrotation were analysed retrospectively,and were compared with the results of the operation and/or the upper gastrointestinal contrast study.Results The positive sign of ultrasound for congenital intestinal malrotation was that the superior mesenteric vein(SMV) being located at the left of the superior mesentery artery(SMA),or being directly anterior to the SMA,and the positive sign for midgut vovulus was clockwise whirlpool sign (clockwise rotation of the small bowel,its mesentery and the SMV around the SMA).The sensitivity of ultrasound for congenital intestinal malrotation was 82％,and the specificity was 94％.Those for midgut vovulus were 94％ and 94％ respectively.Conclusions Special sign as the superior mesenteric vein(SMV) being located at the left of the superior mesentery artery(SMA) strongly indicate congenital intestinal malrotatione,and the clockwise whirlpool sign means great possibility of midgut vovulus.
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Objective To investigate the correlative factors for osteoporosis among senile patients with multiple cardiovascular diseases and type 2 diabetes mellitus.Methods 206 patients were divided into osteoporosis (OP) group and non-osteoporosis (non-OP) group according to the diagnosis standards of osteoporosis.The general data were recorded.Clinical history of all kinds of cardiovascular diseases and type 2 diabetes mellitus were detected.Serum levels of hemoglobin (Hb),glucose (Glu),glucosylated hemoglobin (HbA1c),alkaline phosphatase (ALP),total serum protein (TP),albumin (ALB),BUN,creatinine (Cr),calcium (Ca),phosphonium (P),total cholesterol (TC),triglyceride (TG),high-density lipoprotein cholesterol (HDL-C),low-density lipoprotein cholesterol (LDL-C) were detected.Dual-energy Xray absorptiometry was used to detect the bone mineral density (BMD) of bilateral femoral neck and lumbar spine.The related factors of BMD were analyzed.Results The morbidity of osteoporosis was much higher in elderly females than in elderly males (58.7％ vs.38.6％).The osteoporosis patients were older than non-osteoporosis patients in both males and females,although there was no significant difference.The body mass index (BMI) of both gender was much lower in osteoporosis group than in non-osteoporosis group (P＜0.05).In male patients,BMD was positively correlated with BMI significantly (P＜0.01).In female patients,TC and LDL C were higher in OP group than in non-OP group (P＜0.05).In female patients,BMD was positively correlated with BMI,and negatively correlated with TC and LDL-C.Logistic regression analysis showed that the lower BMI was an independent risk factor for osteoporosis in male patients; BMI and TC were independent risk factors for osteoporosis in female patients.Conclusions In both male and female,senile patients with lower BMI,multiple cardiovascular diseases and type 2 diabetes mellitus have higher risks for osteoporosis.In senile female patients,TC may be correlated with BMD.TC should be reduced to decrease the risks of cardiovascular diseases and osteoporosisin senile females.
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Objective To investigate the quality of life (QOL) of hemodialysis patients and its influencing factors.Methods One hundred and twenty-five patients who underwent maintenance hemodialysis treatment for more than 3 months were selected,and their age,gender,combined disease and dialysis status were recorded.Hemoglobin,albumin and urea clearance index (Kt/V) were tested.Charlson complications index,Karnofsky activity index,Hamilton Depression Scale and MOS 36-item short health survey questionnaire were used to assess complication,activity,and psychological situation of the patients.Results Of the 125 patients,there were 7 (5.6％) grade 1 patients (could not take care of themselves),43 (34.4％) grade 2 patients (could only take care of themselves),47 (37.6％) grade 3 patients,and 13 (10.4％) grade 4 patients.A significant relationship between rehabilitation status and age was found.Young patients were better than older patients (x2 =5.864,P ＜ 0.05).The majority of grade 1 patients were older and had been diagnosed with diabetes,and their Karnofsky activity index,Charlson complications index and Hamilton Depression Scale index were lower than Grade 2-4 patients(t values were 5.263,7.866,6.215 and 5.474,respectively; all P ＜ 0.05).The QOL of the patients were related with age,diabetes,dialysis,serum albumin level,activity index,index of Charlson complications and Hamilton depression (r =-0.618,-0.512,0.593,0.369,0.801,-0.696 and-0.516,respectively).Conclusions The QOL of maintenance hemodialysis patients may be related with physical,psychological,and many other factors.It should be needed to strengthen health care and emotional support to the patients.
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ObjectiveTo evaluate arterial stiffness and its influencing factors in the elderly patients with hypertension and (or) type 2 diabetes mellitus.MethodsTotally 320 elderly patients were divided into four groups: control,diabetes mellitus,hypertension,coexisting hypertension and diabetes mellitus.Arterial stiffness in ankle and brachial artery was determined by brachial-ankle pulse wave velocity (baPWV).Other factors affecting baPWV were also collected and measured,including age,body mass index (BMI),sex,smoking,blood pressure,pulse pressure,mean arterial pressure,serum fasting blood glucose,lipid profile,glycosylated hemoglobin and hypersensitive C-reactive protein (hsCRP).ResultsbaPWV in the four groups were ( 1667.2 ± 279.3 )cm/s,(2158.6 ±386.9)cm/s,(1881.2±383.8) cm/s and (2165.9± 479.9)cm/s,respectively.The baPWV value was higher in groups of hypertension,diabetes mellitus,and the coexisting diseases than in controls (F=8.473,P＜0.05).Diabctic patients had a higher baPWV levels than those without diabetes.There was no difference in baPWV levels between diabetic patients with or without hypertension.Pulse pressure and hsCRP were higher in coexisting diseases group than in control group (P＜0.05).Stepwise backward regression analysis showed that fasting blood glucose,pulse pressure,mean blood pressure,hsCRP and low density lipoprotein cholesterin (LDL-C) were positively related to baPWV P ＜0.05). ConclusionsHypertension and (or) type 2 diabetes mellitus are important influencing factors for higher arterial stiffness. Hyperglycemia might play more important role in the elderly arterial stiffness.Serum fasting blood glucose,pulse pressure,mean blood pressure,hsCRP,LDL-C may be the independent risk factors of arterial stiffness.
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Objective To investigate the effects of multifactor intervention on the rehabilitation outcomes of stroke patients. Methods A total of 85 stroke patients were randomly assigned to the treatment group (receiving intervention plan assessment before undergoing multifactor interventions) and the control group ( receiving conventional rehabilitation). Results The effectiveness of the treatment or control group was 95.3% and 83. 3%, respectively. After the intervention, the patients' potential and alternative risk factors were significantly decreased,although clinical efficacy was largely increased (P ＜0. 01 ). Conelusion Multifactor intervention on elderly stroke patients may be an effective treatment method to control the risk factors and recurrence of stroke.
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Objective To investigate the effect of group psychotherapy on depression in the elderly. Methods A total of 82 elderly patients with depressive disorder were enrolled and assigned to the control group ( n = 40 ) and therapy group ( n = 42 ). The control group obtained general psychological guide and drug therapy, while the therapy group received group psychotherapy and anti-depressants for 4 weeks. One week before and after the intervention, Hamilton depression scale (HAMD) and synthetic life quality scale were used to estimate the functions of the participants. Results At 4 weeks, total score of HAMD of the therapy group was significantly lower than that of the control group (P＜0. 01 ). Synthetic life quality score showed that body, mental and social functions and life quality of the therapy group were significantly higher than the control group. Conclusion Group psychotherapy could be an effective and acceptable treatment for depression in the elderly.
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Objective To observe the efficacy of continuous renal replacement therapy (CRRT) treatment in multiple organ dysfunction syndrome (MODS),and explore its application value.Methods Forty-six cases of MODS were treated with CRRT.Blood gas analysis,toxin removal,electrolyte and APACHE Ⅱ score was observed before and after treatment.Results The APACHE Ⅱ score in the beginning of treatment was (20.8 ±4.4) scores,31 cases had clinical cure,and APACHE Ⅱ score was (13.6 ±4.9)scores,15 cases died.In patients of survival,blood pressure,blood gas analysis,electrolytes,toxin removal improved gradually,and the difference had statistical significance before and after treatment.In 8 cases withshock before treatment who need drugs to maintain blood pressure,after treatment 6 cases withdrew drugs gradually.In the course of treatment,heart function was stable in patients who had heart failure.Four cases had pulmonary infection-induced MODS.In the process of the treatment internal environment was stable,and urine after treatment improved in different degrees.Two cases died of drug-resistant pathogens ultimately.Conclusions CRRT is an effective method in the treatment of MODS patients.CRRT can effectively clear the metabolites and inflammatory mediators,and rebuild the immune homeostasis of the body.
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OBJECTIVE: To explore the mechanism of Chaiqin Chengqi Decoction (CQCQD) in treatment of rats with acute necrotizing pancreatitis (ANP). METHODS: Thirty SD rats were randomly divided into 3 groups: sham-operated (SO) group, ANP group and CQCQD-treated group. ANP was induced by retro-pumping 3.5% sodium cholate to common bile duct. Blood sample was collected from abdominal vein for examination and the pancreatic tissue samples were taken for making pathology section 6 hours later. The pancreatic tissue (HE staining) was observed by light microscope. The content of tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) was detected with the method of enzyme-linked immunosorbent assay, and the activation of nuclear factor-kappaB (NF-kappaB) in pancreas was detected by immunohistochemical method. RESULTS: Compared with the SO group, there was dramatic increase in the white blood cell (WBC) counts and AMY level in the ANP group (P<0.05, P<0.01). Compared with the ANP group, the WBC counts and AMY level in CQCQD-treated group were significantly reduced (P<0.05). The edema, inflammatory infiltration, haemorrhage and necrosis scores and total pathological score in the ANP group were obviously higher than those in the SO group (P<0.01). The edema, haemorrhage and inflammatory infiltration scores and the total pathological score in CQCQD-treated group were decreased (P<0.05). The integral optical density of NF-kappaB p65 positive cells of pancreas in CQCQD-treated group was lower than that in the ANP group (P<0.05). CONCLUSION: CQCQD can reduce the content of serum TNF-alpha and IL-6, depress the activation of NF-kappaB, and lessen the pancreatic lesions.
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Objective To investigate therapeutic autcomes of using telomerase inhibitors to treat cancer at the presumably most and least opportune circadian stages basing on our earlier study. Methods Twenty-four BALB/C nude mice were synchronized to a regimen of LD12:12 for 4 wk. Hepatic cancer cells (SMMC-7721) were implanted into both flanks of each mouse.Two weeks after transplantation,the hTERT-5'RZ was used to treat the hepatic cancer transplanted into the nude mice daily for two weeks,the injection times being either 9 or 21 HALO.Results The tumorinhibition ratio of mice treated at 21 HALO (65%) was statistically significantly higher than that of mice treated at 9 HALO (48%). Telomerase activity was also reduced to a greater extent in mice treated withhTERT-5'RZ at 21 than at 9 HALO, that was at the time of maximal circadian telomerase activity. Conclusion Injection of ribozyme targeted to telomerase during the tumor's DNA synthesis is associated with a betterinhibition of tumor growth and a better therapeutic outcome in hepaticcancer.
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Study of mechanism of medicine actions, by quantitative analysis of cultured cardiac myocyte, is one of the cutting edge researches in myocyte dynamics and molecular biology. The characteristics of cardiac myocyte auto-beating without external stimulation make the research sense. Research of the morphology and cardiac myocyte motion using image analysis can reveal the fundamental mechanism of medical actions, increase the accuracy of medicine filtering, and design the optimal formula of medicine for best medical treatments. A system of hardware and software has been built with complete sets of functions including living cardiac myocyte image acquisition, image processing, motion image analysis, and image recognition. In this paper, theories and approaches are introduced for analysis of living cardiac myocyte motion images and implementing quantitative analysis of cardiac myocyte features. A motion estimation algorithm is used for motion vector detection of particular points and amplitude and frequency detection of a cardiac myocyte. Beatings of cardiac myocytes are sometimes very small. In such case, it is difficult to detect the motion vectors from the particular points in a time sequence of images. For this reason, an image correlation theory is employed to detect the beating frequencies. Active contour algorithm in terms of energy function is proposed to approximate the boundary and detect the changes of edge of myocyte.